PROFILING GC-MS ETHANOL EXTRACT OF PUTRI MALU LEAVES (Mimosa pudica L.) AND IT'S POTENTIAL AS ANTICHOLESTEROL IN VITRO

Abstract

Cholesterol plays a crucial role in cell building and hormone production in the body, but
excessive levels can lead to various diseases. Elevated cholesterol levels increase the risk of
cardiovascular diseases such as atherosclerosis. Natural remedies for high cholesterol treatment
are commonly sourced from medicinal plants containing many phytochemicals. One such plant is
the Putri Malu (Mimosa pudica L.) leaves . This study aims to evaluate the chemical composition
of Putri Malu (Mimosa pudica L.) leaves extract and its potential anti-cholesterol effects in vitro.
The extraction process used in this study was maceration, followed by GC-MS profiling analysis
and the Liebermann Burchad method with UV-Vis spectrophotometry. The findings of this study
revealed the presence of five chemical constituents in the extract. Among them, the second and
fourth peaks had the highest abundance of acacetin at 14.08% and diosmetin at 73.79%,
respectively. The ethanol extract of Putri Malu leaves demonstrated strong anti-cholesterol
activity in vitro with an IC50 value of 24.8993, indicating its potential as an agent for treating
hypercholesterolemia. Further research is required to evaluate this extract's efficacy in vivo and
investigate its underlying mechanism of action.

Keywords: Anti-cholesterol, extract of putri malu leaves, GC-MS profiling analysis, Liebermann
Burchard, maceration