Antibacterial Activity Testing on APMS (p-Methoxy Cinnamic Acid) Against Escherichia coli Bacteria

Abstract

This study aims to determine whether the compound APMS (p-methoxy cinnamic acid) has the
antibacterial activity of Escherichia coli and to determine the effective concentration of the
compound APMS (p-methoxy cinnamic acid) in inhibiting the growth of Escherichia coli bacteria.
This research begins with the synthesis of APMS obtained through the knoevenagel condensation
reaction with the sonochemical method. The synthesized compounds were tested organoleptically
and their melting points were measured. The structure of the results was elucidated using FT-IR
and GC-MS, then tested the antibacterial activity of APMS compounds against Escherichia coli.
Data analysis with SPSS, 99% confidence level (p<0.01) and continued with ANOVA test. The
compound synthesized by APMS is in the form of fine crystals with a glossy white color, has a
characteristic odor, and produces a % yield of 92.71%. The level of p-methoxy cinnamic acid
(APMS) produced from this study was 95% using the GC-MS Instrument. APMS compounds have
antibacterial activity against Escherichia coli. The average inhibition zone formed at a
concentration of 5%; 10%; and 15% respectively are 0.695 cm; 0.727 cm; and 0.855 cm The optimal
concentration of this study was at a concentration of 15% which gave the greatest inhibition
Keywords: Antibacterial; Escherichia coli; p-Methoxy cinnamic acid