Anti-inflammatory Activity of Salicylanilide Compounds from Gondopuro Oil as an In-Vitro

Abstract

A component of gondopuro oil is methyl salicylate. Methyl salicylate has functional
groups that allow it to be changed. Changes in functional groups can occur through chemical
reactions, one of which is through the aminolysis reaction to produce salicylamide or salicylanilide.
The precursor for the synthesis of amide derivatives is a carboxylic acid, using natural methyl
salicylate from gondopuro oil and amines sonochemically using sonichemical methods for 3, 4, and
5 hours with temperature control of ± 60ºC. Then liquid extraction was carried out using of hexane
and distilled water 2 to 3 times until 2 phases were formed, then left for 1 night at a temperature of
less than 10^o C and decanted. Then extracted with 10 mL of cold 5% NaOH 2 times until two phases
were formed, namely the n-hexane fraction and the NaOH fraction. The NaOH fraction obtained is
then heated using a water bath to evaporate the solvent so that it becomes a solid, which is then
placed in an oven to form crystals. The crystals obtained were then dried using an oven at 40ºC
until dry, and then the yield of the synthesized powder was calculated. From the research results, it
can be concluded that Salicylanilide compounds can be synthesized using sonochemical methods
and produce the largest % yield in the 3rd sample at 5 hours at 11.23%. Salicylanilide compounds
have anti-inflammatory activity at a concentration of 100.0 ppm with a % inhibition of 46.07%.

Key word: Anti-inflammatory, Gondopuro oil, Inhibition, Salicylanilide, Sonochemistry