Review of Isolation Methods, Chemical Composition and Biological Activities of Curcuma aeruginosa Roxb Rizhome

Abstract

Curcuma aeruginosa Roxb rhizome is known as temu ireng or temu hitam in Indonesia and has
been widely used as a traditional medicine in Asia. There have been many studies to reveal the
chemical content of Curcuma aeruginosa Roxb rhizome, some of which are influenced by the
isolation methods. Several separation and purification methods were identified including
essential oil distillation, extraction and chromatography. This study aims to describe, analyze
and discuss the isolation methods of chemicals compounds Curcuma aeruginosa Roxb rhizome,
how the techniques influence the types and composition of the components and to examine
which compounds have potential biological activities. The method used for review articles is
explored several databases such as PubMed and Scopus to identify and download abstracts,
original articles, and research papers related to extraction followed by fractionation, isolation,
and chemical components of Curcuma aeruginosa Roxb. The inclusion criteria used were the
article year between 2010-2020, the part used for the study was the rhizome, there was a
discussion of isolation process, components that have been isolated and their biological activity.
The exclusion criteria used were articles whose samples were only up to the extraction stage and
articles whose methods were unclear. Chemical compounds of Curcuma aeruginosa Roxb
rhizome can be carried out in various ways, including steam distillation, water distillation,
extraction, fractionation to isolation by chromatography, especially column chromatography
with elution gradient. Germacron is the most isolated and has several biological activities.

Keywords: Curcuma aeruginosa Roxb, Rhizome, Isolation, Chemical composition, biological
activity.