The Effect of Aqueous Fraction of Clinacanthusnutans (Burm.F.) Extract Microcapsules on Rat Blood Glucose Level

Abstract

Diabetes mellitus is a common metabolic disease marked by
hyperglycemia which is also caused by oxidative stress. The administration of
antioxidants coming from food or supplements can inhibit the formation of
free radicals. Clinacanthus nutans has been reported to possess various
medicinal advantages such as blood glucose lowering effect, alphaglucosidase

inhibition activity, antioxidant activities, anti-cancer properties,
and anti-inflammatory effects. However, C. nutans leaf extracts fractions has
several limitations, i.e. highly viscous that may constraint its pharmaceutical
formulation as well as the astringent and bitter taste which diminish its oralbased

formulation. In order to solve this problem, microencapsulation
strategy during formulation can be used here in. The purpose of this research
is to find the blood glucose level lowering effect of the aqueous fraction of
microcapsules and to find the effective dose on fructose and fat-induced
Wistar strain rat. The induction was done using fructose and high-fat feed for
55 days. Rats were divided into 8 groups: normal control (aquadest), negative
control (Arabic gum and maltodextrin), positive control (metformin), dose I
(15.89 mg/kg BW microcapsule of aqueous fraction of Clinacanthus nutans
extract), dose II (31.78 mg/kg BW), dose III (47.67 mg/kg BW), dose IV (63.56
mg/kg BW), and dose V (79.45 mg/kg BW). The result showed that the
microcapsules of the aqueous fraction of Clinacanthus nutans (Burm.f) extract
could lower Wistar strain rat blood glucose level in the dose of 15.89 mg/kg
of the rat’s body weight.
Keywords: Clinacanthus nutans, diabetes mellitus, fructose, high-fat feed.