Characteristics of Nanoparticle Caulerpa Racemosa as an Anti-Breast Cancer Agent

Abstract

Caulerpa racemosa is a marine algae rich in folic acid, thiamine, and
caulerpenyne, characterized by potential anticancer, antitumor and antiproliferative
properties.
Despite
the
promising
potential,
the
clinical
application
of
natural

ingredients
often
faces
challenges
due
to
low
bioavailability.
To
address
this
limitation,
nanotechnology
provides
a
promising
avenue
for
enhancing
bioavailability.
Therefore,
this
research
aimed
to
investigate
the
physical
characteristics,
including
particle
size
and
zeta
potential,
of
nanoencapsulated
Caulerpa
racemosa

extract
at
three
dosage
levels
of
100
mg/mL,
125
mg/mL,
and
150
mg/mL.
The

extraction
process
entailed
maceration
for
3x24
hours,
resulting
in
nanoparticle

formed
through
the
combination
of
Caulerpa
racemosa
extract
with
tween
80,
a

0.3%
chitosan
solution

in acetate buffer (pH 4), and a tripolyphosphate (TPP)
solution in distilled water. Particle size analysis using Particle Size Analyzer
showed that the nanoencapsulated extract had larger particle sizes in the nano
range, while phytochemical screening confirmed the presence of flavonoids, alkaloids,
terpenoids,
and
phenolics.
Zeta
potential
and
polydispersity
index
measurements
showed
that
higher
dosage
levels
led
to
smaller
zeta
potential
values,

although

remaining below +30 mV. Specifically, the polydispersity index was
highest at a dosage level of 125 mg/mL.
Keywords: Caulerpa Racemose Extract, Nanoparticle, Physical Characteristics,
Anticancer