LEAF EXTRACT MICROENCAPSULATION of Stevia rebaudiana Bert USING INULIN-CHITOSAN AS ANTI-DIABETES DIET
Abstract
ABSTRACT
Diabetes mellitus is a collection of symptoms that arise in someone who has increased blood
glucose levels. The Stevia plant (Stevia rebaudiana Bert) contains a compound of diterpene
glycosides as steviosida and rebaudiosida A. Purified extract of steviosida and rebaudiosida A is
widely used as a sweetener for low calorie food and beverage products or as a sugar substitute for
diabetics and has the effect of lowering blood sugar levels. This study aims to determine the
antidiabetic effect of microencapsulated preparations of Stevia leaf extract (Stevia rebaudiana)
with a combination of inulin chitosan encapsulation. Antidiabetic mellitus test was carried out in
vivo using test animals of male white rats of Wistar strain. The inducing compound that can cause
the condition of diabetes mellitus test animals is Aloxan with a dose of 150 mg / kg Body weight
of rats. given intraperitoneally for one day, then the mice were allowed to stand for 3 days to reach
a state of diabetes mellitus. Blood glucose levels of test animals were measured on days 1 (initial),
5 (induction) and 12 (treatment) to determine the initial blood glucose levels, after induction of
alloxan and after administration of test compounds both CMC Na 0.5% , glibenclamide, and
preparations microencapsulation of Stevia leaf extract at a dose of 100; 300; and 700 mg / kg
body weight. The results were obtained after 7 days of treatment, it was seen that blood glucose
levels in the CMC group remained high, while the Glibenclamid administration group, and the
three dosages of microencapsulation preparations of Stevia leaf extract could reduce blood
glucose levels. This can be seen from the statistical test that there is a significant difference (p
<0.05) between the CMC group and the Glibenclamide group, and microencapsulation
preparations of Stevia leaf extract. Microencapsulation preparations of Stevia leaf extract can
reduce blood glucose levels with an effective dose of 100 mg / kg body weight.
